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Imaging cells with the atomic force microscope.

H J Butt1, E K Wolff, S A Gould

  • 1Department of Physics, University of California, Santa Barbara 93106.

Journal of Structural Biology
|October 1, 1990
PubMed
Summary
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Atomic force microscopy (AFM) reveals detailed cell surface structures. This technique provides high-resolution imaging of various cells, including bacteria and blood cells, even under physiological conditions.

Area of Science:

  • Cell biology
  • Microscopy
  • Biophysics

Background:

  • Traditional microscopy methods face limitations in resolving nanoscale surface features of biological specimens.
  • Imaging cells under physiological conditions is crucial for understanding their natural behavior and function.

Purpose of the Study:

  • To demonstrate the capability of atomic force microscopy (AFM) for high-resolution imaging of diverse cell types.
  • To showcase AFM's utility in visualizing cellular morphology under various environmental conditions, including physiological states.

Main Methods:

  • Atomic Force Microscopy (AFM) was employed to image various biological samples.
  • Samples included the archaebacterium Halobacterium halobium, a stoma from Lagerstroemia subcostata, a water lily leaf, and fixed red and white blood cells.

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Main Results:

  • High-resolution images of Halobacterium halobium in a dry state were obtained.
  • Surface topography of a plant stoma and the underside of a water lily leaf (down to 12 nm) were visualized.
  • Detailed imaging of red and white blood cells (down to 8 nm) was achieved in a buffer solution.

Conclusions:

  • Atomic force microscopy (AFM) is a powerful tool for high-resolution surface imaging of cells.
  • AFM enables the visualization of cellular structures under physiologically relevant conditions, offering insights into cell morphology and surface characteristics.