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Circular dichroism detection in high-performance liquid chromatography: evaluation of the anisotropy factor
C Bertucci1, E Domenici, P Salvadori
1Centro di Studio del C.N.R. per le Macromolecole Stereordinate ed Otticamente Attive, Dipartimento di Chimica e Chimica Industriale, Universitá di Pisa, Italy.
Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1990
Abstract:
The application of a circular dichroism (c.d.) detection system in HPLC using a chiral stationary phase is presented. The simultaneous measurement of the absorbance and c.d. signal allows the evaluation of the anisotropy factor (g = delta epsilon/epsilon) and thus the determination of the enantiomeric excess (e.e.) of the eluates. When this detection system is used in preparative chiral chromatography the collection of the enantiomeric fractions can be readily optimized.