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Characterization of an intermediate adenovirus strain
1Institute of Microbiology, Semmelweis University Medical School, Budapest, Hungary.
Acta Microbiologica Hungarica
|January 1, 1990
Summary
This study identified an intermediate human adenovirus strain using serological and genetic methods. Both serum neutralization and restriction endonuclease analysis confirmed it as human adenovirus type 9.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Human adenoviruses (HAdVs) are common viral pathogens with diverse serotypes.
- Subgenus D adenoviruses present unique characteristics and require precise identification.
- Understanding adenovirus strain variations is crucial for diagnostics and epidemiology.
Purpose of the Study:
- To characterize an intermediate strain of human adenovirus belonging to subgenus D.
- To determine the precise serotype of the investigated adenovirus strain.
- To compare the efficacy of different identification methods for adenovirus strains.
Main Methods:
- Type-specific serological reactions, including serum neutralization and haemagglutination inhibition tests.
- Restriction endonuclease analysis (REA) for genetic fingerprinting.
- Comparison of results from serological and molecular methods.
Main Results:
- Restriction endonuclease analysis identified the strain as identical to the prototype human adenovirus type 9.
- Serum neutralization tests also confirmed the strain's identity as human adenovirus type 9.
- Haemagglutination inhibition tests indicated a relationship with both human adenovirus types 9 and 13.
Conclusions:
- The intermediate adenovirus strain is definitively identified as human adenovirus type 9.
- Discrepancies between haemagglutination inhibition tests and other methods highlight the need for multiple diagnostic approaches.
- Accurate adenovirus strain identification is essential for clinical and public health applications.