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Conditions required for induction of murine p30 by herpes simplex virus
Abstract:
Mouse cells (line N cIA cl10) contain 1.2-2.5 ng murine leukaemia virus (MuLV) p30 antigen/mg of protein; this amount of antigen is measurable by competition radioimmunoassay (RIA) but is not detectable by indirect immunofluorescence (IF). Infection of N cIA cl10 cells with herpes simplex virus type 2 (HSV-2) induces expression of MuLV p30. Induction by HSV-2 does not require either cell or virus DNA synthesis and is optimal 8 h post infection when cells at 50-70% confluence are infected at a multiplicity of infection (MOI) of 5-8 PFU/cell. At an MOI of 2.5, 70-80% of the cells express HSV antigens while none of the cells express p30; at an MOI of 5.0, 70-80% of the cells express HSV antigens but 55% of the cells express p30. Using the conditions reported in this paper for preparation of competing antigen, induction of p30 by HSV-2 (strain 333) infection is not measurable by competition RIA.
Insights
Herpes simplex virus type 2 (HSV-2) infection induces murine leukemia virus (MuLV) p30 antigen expression in mouse cells. However, this induction is not detectable by radioimmunoassay under specific conditions.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Murine leukemia virus (MuLV) p30 antigen is present in N cIA cl10 mouse cells.
- This p30 antigen is detectable by radioimmunoassay (RIA) but not indirect immunofluorescence (IF).
Purpose of the Study:
- To investigate the induction of MuLV p30 antigen expression in mouse cells by herpes simplex virus type 2 (HSV-2).
- To determine the optimal conditions for HSV-2 induced MuLV p30 expression and its detectability.
Main Methods:
- Infection of N cIA cl10 mouse cells with HSV-2 at various multiplicities of infection (MOI).
- Analysis of MuLV p30 antigen expression using immunofluorescence and competition radioimmunoassay.
- Assessment of the role of cell and virus DNA synthesis in the induction process.
Main Results:
- HSV-2 infection induces MuLV p30 expression in N cIA cl10 cells.
- Induction is optimal at 8 hours post-infection with an MOI of 5-8 PFU/cell.
- At an MOI of 5.0, 55% of cells express p30, while at lower MOI, no p30 is detected.
- MuLV p30 induction by HSV-2 (strain 333) was not measurable by competition RIA under the reported antigen preparation conditions.
Conclusions:
- HSV-2 infection can trigger the expression of MuLV p30 antigen in mouse cells.
- The induction process is independent of DNA synthesis and is dose- and time-dependent.
- Specific experimental conditions for antigen preparation can affect the detectability of induced p30 by RIA.