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Plasmid resistance to ampicillin in Salmonella typhi
M Marranzano1, M D'Angelo, A Agodi
1Istituto di Igiene e Medicina Preventiva, Universitá di Catania.
Bollettino Dell'Istituto Sieroterapico Milanese
|January 1, 1990
Summary
A multi-drug resistant Salmonella typhi strain was identified in Catania in 1988. This resistance was linked to a transferable R-plasmid encoding resistance to ampicillin, streptomycin, and sulfonamides.
Area of Science:
- Microbiology
- Genetics
- Epidemiology
Background:
- Salmonella typhi is a significant human pathogen.
- Antimicrobial resistance poses a growing global health threat.
- Understanding resistance mechanisms is crucial for effective treatment.
Purpose of the Study:
- To characterize a multi-drug resistant Salmonella typhi strain.
- To identify the genetic basis of antimicrobial resistance in this isolate.
Main Methods:
- Bacteriological isolation and identification.
- Antimicrobial susceptibility testing.
- Plasmid isolation and characterization (molecular weight, incompatibility group determination).
Main Results:
- A Salmonella typhi strain exhibiting resistance to ampicillin, streptomycin, and sulfonamides was isolated.
- The observed resistances were encoded by a self-transferable R-plasmid.
- The R-plasmid had a molecular weight of 70 Md and belonged to incompatibility group B.
Conclusions:
- The study identified a transferable R-plasmid as the mechanism for multi-drug resistance in a Salmonella typhi isolate.
- This finding highlights the role of plasmids in the dissemination of antimicrobial resistance.
- Characterization of such plasmids is essential for monitoring and controlling resistant bacterial strains.