Optimum pH and ionic strength for the assay of cytochrome c oxidase from pea cotyledon mitochondria

Cytochrome c oxidase (EC 1.9.3.1) has been solubilized by use of the nonionic detergents were determined for the oxidation of ferrocytochrome c in air. The results indicate that the plant cytochrome c oxidase resembles mammalian preparations in its sensitivity towards ionic strength and pH of the assay buffer.