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Cryosectioning Method for Microdissection of Murine Colonic Mucosa
Published on: July 12, 2015
Structural, histochemical and immunocytochemical study of the forestomach mucosa in domestic ruminants
G Scala1, M Corona, L Maruccio
1Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via Veterinaria, 1, 80137 Naples, Italy. gaescala@unina.it
Abstract:
The forestomach plays an important role in the digestion physiology of ruminants. The aim of this study is to clarify the morpho-functional role of the mucosa in each of the three compartments of the forestomach in three domestic ruminants species, viz cattle, buffalo and sheep, by means of structural, histochemical and immunocytochemical methods, including transmission electron microscopy, light microscopy and scanning electron microscopy. These methods were chosen to demonstrate the indirect evidence for the presence of nitric oxide (NO) employing NADPHd and nitric oxide synthase I (NOS I). The various cell layers of the forestomach epithelium are described and illustrated in detail. An intense NADPHd staining was observed in the granulosa, spinosa and basal layers of the epithelium, in particular in the cytoplasm over the nucleus. NOS I immunoreactivity was found in all specimens of the forestomach mucosa. The results of this study might reflect a possible role of NO in delaying the onset of cellular apoptosis in the forestomach mucosa of the domestic ruminants, by playing a role in the production of cell energy.
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