A new chromatographic response function for use in size-exclusion chromatography optimization strategies: Application...

A new chromatographic response function (CRF) is presented aiming at designing an optimal chromatographic separation protocol for assessing the molecular size distribution of complex organic mixtures, such as those of natural organic matter from different sources (atmospheric, aqueous, and terrestrial). This CRF can be applied to mixtures of unknown solutes, being well suited for describing separation processes of pair of peaks of highly unequal area, and also for overlapping and asymmetric peaks. The performance of the developed CRF was compared to that of an existing response function, using simulated chromatograms. The capability of the new function to qualify the resolution degree that it is attained under different chromatographic conditions was further assessed through a size-exclusion chromatography study of a variety of different organic compounds, via a two-level full factorial design. It was proved that this function is a reliable alternative to optimize simultaneously the composition of the mobile phase (pH, ionic strength, and organic modifier concentration) and the instrumental variables (flow rate).