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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Fabricating and Labeling Microbubbles with Fluorescent and Radioactive Tracers
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Fabricating and Labeling Microbubbles with Fluorescent and Radioactive Tracers

Published on: January 24, 2025

On sizing and counting of microbubbles using optical microscopy.

Charles A Sennoga1, Veronique Mahue, Jonathan Loughran

  • 1Department of Bioengineering, Imperial College London, South Kensington Campus, London, United Kingdom. c.sennoga@imperial.ac.uk

Ultrasound in Medicine & Biology
|October 30, 2010
PubMed
Summary
This summary is machine-generated.

Optical microscopy (OM) assessment of microbubble variability revealed higher intraobserver variations for SonoVue™, likely due to natural decay. This study details a protocol for microbubble sizing and counting.

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Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
06:02

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Published on: June 12, 2021

Area of Science:

  • Microscopy and Imaging Science
  • Biomedical Engineering
  • Materials Science

Background:

  • Accurate quantification of microbubbles is crucial for various biomedical applications, including diagnostic imaging and drug delivery.
  • Optical microscopy (OM) is a common technique for microbubble characterization, but its reliability is subject to observer variability.
  • Understanding and quantifying this variability is essential for standardizing microbubble analysis.

Purpose of the Study:

  • To assess the intra- and interobserver variability in sizing and counting microbubbles using optical microscopy.
  • To evaluate the performance of the optical microscopy system with standardized microspheres and a clinical microbubble agent (SonoVue™).
  • To provide a detailed protocol and identify potential pitfalls in microbubble quantification via OM.

Main Methods:

  • System calibration using standardized mono-disperse and poly-disperse microspheres.
  • Assessment of microbubble counting (C) and arithmetic mean size (MS) by three observers.
  • Evaluation of variability for standardized microspheres and SonoVue™ microbubbles using optical microscopy.

Main Results:

  • For standardized microspheres, intraobserver variations were: mono-disperse (C=13.0%, MS=0.2%) and poly-disperse (C=6.9%, MS=0.8%).
  • Interobserver variations for standardized microspheres were: mono-disperse (C=18.4%, MS=0.6%) and poly-disperse (C=10.5%, MS=0.3%).
  • For SonoVue™, intraobserver variations were higher (C=23.3%, MS=8.0%), while interobserver variations were lower (C=6.8%, MS=3.8%), suggesting bubble decay impacts consistency.

Conclusions:

  • Higher intraobserver variability for SonoVue™ is likely attributable to the natural decay of microbubbles over time.
  • The detailed protocol and identified pitfalls can aid in improving the consistency and reliability of microbubble quantification using optical microscopy.
  • The results are comparable to previous studies and align with known size distributions, validating the approach.