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Mycoplasmas from donkeys and horses in the Sudan
1Department of Microbiology, Faculty of Veterinary Science, Khartoum North, Sudan.
Abstract:
Seventeen isolates (4.27%) were recovered from 398 samples. Twelve isolates (4%) were obtained from 300 donkey nasal swabs, three (4.3%) and two (6.89%) isolates were recovered from 69 horse nasal swabs and 29 mare uterine washings, respectively. Nine isolates were lost during storage at -20 degrees C and the remaining eight were identified as mycoplasmas and their biological, biochemical and serological reactions were investigated. The isolates could be divided into two groups on the basis of glucose fermentation and arginine hydrolysis. The first group neither fermented glucose nor hydrolysed arginine. Organisms in the second group hydrolysed arginine only.
Insights
Researchers identified mycoplasma in equine and donkey samples. Eight isolates were characterized, revealing two distinct groups based on glucose fermentation and arginine hydrolysis, aiding in mycoplasma classification.
Area of Science:
- Veterinary Microbiology
- Animal Health
- Mycoplasma Research
Background:
- Mycoplasmas are significant pathogens in animal populations.
- Understanding their prevalence and characteristics is crucial for disease management.
- Equine and donkey respiratory and reproductive tracts can harbor various microorganisms.
Purpose of the Study:
- To investigate the prevalence of mycoplasma in equine and donkey populations.
- To characterize the biological, biochemical, and serological reactions of isolated mycoplasma strains.
- To classify the isolates based on key metabolic activities.
Main Methods:
- Collection and processing of 398 samples (donkey nasal swabs, horse nasal swabs, mare uterine washings).
- Isolation and identification of mycoplasma.
- Investigation of biological, biochemical (glucose fermentation, arginine hydrolysis), and serological reactions.
- Classification of isolates into groups based on metabolic profiles.
Main Results:
- Seventeen mycoplasma isolates (4.27%) were recovered from 398 samples.
- Eight isolates were successfully characterized after storage.
- Isolates were divided into two groups: one lacking glucose fermentation and arginine hydrolysis, and another hydrolyzing arginine only.
Conclusions:
- Mycoplasma species were detected in the studied equine and donkey populations.
- Metabolic profiling, specifically glucose fermentation and arginine hydrolysis, can differentiate mycoplasma isolates.
- Further characterization is needed to fully identify the species and their pathogenic potential.