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Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

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Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
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Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

Micron-sized pillars for ion-pair reversed-phase DNA separations.

Wim De Malsche1, Lei Zhang, Jeff Op De Beeck

  • 1Department of Chemical Engineering at the VUB (Vrije Universiteit Brussel), Brussels, Belgium. wdemalsc@vub.ac.be

Journal of Separation Science
|October 30, 2010
PubMed
Summary

Researchers explored micron-sized silicon pillar channels for High-Performance Liquid Chromatography (HPLC). This novel approach enables rapid separation of DNA fragments in seconds using microfluidic devices.

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Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
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Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Published on: December 4, 2017

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Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
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Published on: September 20, 2016

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
10:23

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Published on: December 4, 2017

Area of Science:

  • Microfluidics
  • Analytical Chemistry
  • Materials Science

Background:

  • High-Performance Liquid Chromatography (HPLC) is a crucial analytical technique.
  • Developing miniaturized HPLC systems offers advantages in speed and sample consumption.
  • Silicon microfabrication provides a robust platform for creating microfluidic devices.

Purpose of the Study:

  • To investigate the feasibility of constructing micron-sized silicon pillar channels for HPLC applications.
  • To develop a microfluidic chip capable of high-resolution separations.
  • To assess the performance of the silicon pillar channel system for rapid analysis.

Main Methods:

  • Fabrication of micron-sized silicon pillar channels using deep-UV lithography.
  • Integration of a 3-nL on-chip injection system.
  • Utilizing elongated distribution structures for precise sample definition.
  • Evaluation in isocratic ion-pair reversed-phase (RP) mode.

Main Results:

  • Successfully constructed silicon pillar channels with critical sidewall dimensions below 1 µm.
  • Achieved controlled and high-aspect-ratio sample definition.
  • Demonstrated rapid separation of a two-component mixture (300 and 400 base pairs) in just 5 seconds.

Conclusions:

  • Micron-sized silicon pillar channels are feasible for HPLC.
  • The developed microfluidic system enables ultra-fast separations.
  • This technology holds promise for rapid analysis of biomolecules like DNA fragments.