Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants01:18

Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants

Oral anticoagulants are vital tools in preventing and treating blood clotting disorders. This diverse class of medications can be categorized as vitamin K antagonists, exemplified by warfarin, and direct thrombin inhibitors (DTIs), such as dabigatran, as well as factor Xa inhibitors, including rivaroxaban.
Warfarin, a prominent vitamin K antagonist family member, exerts its effect by inhibiting the enzyme VKORC1 (vitamin K epoxide reductase complex 1). By hindering this enzyme, warfarin...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design of Hyperporous Molecularly Imprinted Thin Films for Ultrasensitive Antibody-Free QCM Detection of a Small-Cell Lung Cancer Biomarker.

ACS sensors·2026
Same author

Kolbe radical-initiated electrochemical desulfurization of thioamides under aerobic conditions.

Chemical communications (Cambridge, England)·2026
Same author

Recent Advancements in Nickel-Catalyzed Electrochemical Reductive Cross-Coupling.

ACS organic & inorganic Au·2025
Same author

Regulation of airway fumarate by host and pathogen promotes <i>S. aureus</i> pneumonia.

bioRxiv : the preprint server for biology·2025
Same author

Exploring the Anticancer Potential of Proton Pump Inhibitors by Targeting GRP78 and V-ATPase: Molecular Docking, Molecular Dynamics, PCA, and MM-GBSA Calculations.

International journal of molecular sciences·2025
Same author

<i>In Silico</i> Frontiers Shaping the Next Generation of Transformation Product Prediction and Toxicological Assessment.

Environmental science & technology·2025
Same journal

3D-Printed Structures Versus Drilled Cavities: A Comparison of Microconfinement Methods for Rheological Characterisation of Multicellular Aggregates.

Journal of molecular recognition : JMR·2026
Same journal

Temperature Dependent Motions of N-Terminal Loop in PD-1 Determine the Affinity Towards Nivolumab.

Journal of molecular recognition : JMR·2026
Same journal

AFM Force Volume for Extracellular Vesicle Detection and Membrane Blebbing Analysis Through Mechanical Signature Mapping.

Journal of molecular recognition : JMR·2026
Same journal

Induced Circular Dichroism From the Binding of Achiral Bivalent Ligands to Transthyretin.

Journal of molecular recognition : JMR·2026
Same journal

Design of Multi-Epitope DIVA-Compatible Vaccine Candidate Against Canine Parvovirus 2.

Journal of molecular recognition : JMR·2026
Same journal

Artificial Intelligence Clustering Approach for Force Mapping Analysis of Polyacrylic Acid (PAA)/Polyethylene Oxide (PEO) Polymer Brushes for Biosensor Applications.

Journal of molecular recognition : JMR·2026
See all related articles

Related Experiment Video

Updated: Jun 7, 2026

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases
06:10

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

Published on: December 23, 2013

Warfarin: an environment-dependent switchable molecular probe.

Ian A Nicholls1, Björn C G Karlsson, Annika M Rosengren

  • 1Bioorganic & Biophysical Chemistry Laboratory, School of Natural Sciences, Linnæus University, SE-391 82 Kalmar, Sweden. ian.nicholls@lnu.se

Journal of Molecular Recognition : JMR
|October 30, 2010
PubMed
Summary
This summary is machine-generated.

Warfarin

More Related Videos

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
08:37

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Published on: November 5, 2014

Related Experiment Videos

Last Updated: Jun 7, 2026

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases
06:10

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

Published on: December 23, 2013

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
08:37

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Published on: November 5, 2014

Area of Science:

  • Biochemistry
  • Physical Chemistry
  • Spectroscopy

Background:

  • Warfarin's anticoagulant activity is linked to its complex structure and diverse binding modes.
  • Understanding warfarin's isomeric forms and their environmental interactions is crucial for its application.

Purpose of the Study:

  • To investigate the relationship between warfarin's molecular environment and its isomeric equilibrium.
  • To explore warfarin's potential as an environment-dependent molecular switch for detection.

Main Methods:

  • Theoretical studies
  • (1)H-NMR spectroscopy
  • Steady-state and time-resolved fluorescence spectroscopy

Main Results:

  • Solvent systems influence warfarin's isomeric equilibrium (hemiacetal, open phenol-keto forms).
  • Different warfarin isomers exhibit unique spectroscopic properties.
  • Warfarin acts as a molecular switch, enabling simultaneous detection of different states with distinct fluorescence lifetimes (0.10–5.5 ns).

Conclusions:

  • Warfarin's structure adapts to its environment, creating a responsive molecular switch.
  • This property allows for the examination of warfarin binding domains in various materials.
  • Warfarin can be utilized as a tool to probe molecular-level characteristics like local dielectricity.