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[Effect of diisobutyl phthalate on antioxidase activity and DNA damage in mice]
Ning Ma1, Wenzhong Zhang, Yongquan Feng
1Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China. ma_ning34@yahoo.com.cn
Objective:
To investigate the oxidative damage of mice induced by diisobutyl phthalate (DiBP) and the mechanism of free radical oxidative damage caused by DiBP.
Methods:
Sixty KunMing mice were divided by weight into 5 groups after accommodation to the experimental animal room for 3 days. The control group and 4 DiBP groups, group I, II, III and IV, were given DiBP in corn oil by gavages at the dosage of 0, 50, 250, 500 and 1000 mg/kg respectively. The mice were fed with normal diets and drinking water freely for 8 weeks. By the end of the experiment, the comet assay of blood and SOD, GSH-Px, MDA and 8-OHdG of liver were tested.
Results:
The activities of SOD and GSH-Px in DiBP groups were significantly lower than the control group (P < 0.05); the MDA contents of DiBP group III and group IV were significantly higher than the control group (P < 0.05) and the 8-OHdG content of group II was significantly higher than the control group (P < 0.01). The comet assay showed that the oxidative damage of DNA in DiBP groups was significant in comparison with the control group (P < 0.05).
Conclusion:
Oxidative stress induced by diisobutyl phthalate can decrease the activities of antioxidative enzymes and result in oxidative damage of tissues.
Insights
Diisobutyl phthalate (DiBP) exposure in mice caused oxidative stress, decreasing antioxidant enzyme activity and damaging DNA. This study reveals DiBP
Area of Science:
- Toxicology
- Environmental Health
- Biochemistry
Background:
- Diisobutyl phthalate (DiBP) is a common plasticizer.
- Phthalates are suspected endocrine disruptors and can cause oxidative stress.
Purpose of the Study:
- To investigate the oxidative damage induced by DiBP in mice.
- To elucidate the mechanism of free radical oxidative damage caused by DiBP.
Main Methods:
- KunMing mice were gavaged with 0, 50, 250, 500, or 1000 mg/kg DiBP for 8 weeks.
- Blood comet assay and liver assays for SOD, GSH-Px, MDA, and 8-OHdG were performed.
Main Results:
- DiBP exposure significantly decreased SOD and GSH-Px activities.
- MDA and 8-OHdG levels increased in a dose-dependent manner.
- DNA oxidative damage was observed via comet assay.
Conclusions:
- DiBP induces oxidative stress by reducing antioxidant enzyme activity.
- DiBP causes tissue damage through oxidative stress pathways.
