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[Expression and clinical significance of Ran in lung squamous carcinoma tissues]
1Department of Occupational Medicine, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Objective:
To investigate the expressions on mRNA and protein levels and clinical significance of Ras-related nuclear protein (Ran) in lung squamous carcinoma tissues.
Methods:
Expressional levels of Ran were detected by the methods of real-time quantitative PCR and Western Blot consisting of 22 cases of lung squamous carcinoma tissues and adjacent normal tissues with different TNM stages and differentiated degree, then the correlations of Ran expressional levels to pathological characters were evaluated.
Results:
The mRNA expression of Ran in squamous cell carcinoma tissues was 2.35 times of that in the adjacent normal tissues and the difference was significant (P < 0.05). The expressional level of Ran protein in lung squamous carcinoma tissues was higher than that in the adjacent normal tissues. The expressional level on mRNA and protein in III stage tissues was higher than that of I and II stages in lung squamous cell carcinoma (P < 0.05). The difference of expression level between differentiated degree was not found (P > 0.05).
Conclusion:
The increased expression of Ran in lung squamous cell carcinoma might be correlated with carcinogenesis and used as a supporting marker for early diagnosis of lung carcinoma.
