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[Analysis on opioid compounds in the hair of heroin addicts with LC-MS/MS]
Jianxin Zhang1, Pinjia Meng, Daming Zhang
1Drug Intelligence and Forensic Center of the Ministry of Public Security, Beijing 100193, China. zhangjianxin7292000@yahoo.com.cn
Objective:
To evaluate the effects of washing process on hair samples from heroin addicts, and compare the extracting method of enzymatic digestion with homogenization of hair samples.
Methods:
After washing samples for 3.15 h, a mild enzymatic digestion procedure at pH 6.6 and a buffer incubating procedure in water bath at 48 degrees C during 18h was used. The samples were pre-treated by solid phase extraction using mixed mode sorbent columns (MCX Oasis). Quantitative analysis of morphine, codeine, 6-acetylmorphine, heroin, acetylcodeine by chromatography-tandem mass spectrometry method was developed.
Results:
Heroin accounted for 21.82% of the total equivalents of morphine. The ratio of morphine to 6-MAM in enzymatic digestion hair samples was 0.9875 and that in homogenized hair samples was 0.3948.
Conclusion:
The results suggested that the procedures were not sufficient to remove the contaminants penetrating into hair from external sources.
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