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[Some bio-chemical indice change of rats striatum poisoned by rotenone]
Hui Liu1, Fangqiu Yin, Jianmei Zhang
1Department of Health Service, Norman Bethune Military Medical College, Shijiazhuang 050081, China.
Objective:
To observe some of bio-chemical indice of rats striatum poisoned by rotenone.
Methods:
Apply the method of back-implantation mini-effusion pump to observe influence of different concentration rotenone on the rat striatum. Utilize Fluoro-Jade B combined Fluorescent dying to observe the change conditions of the neural cells of the poisoned rat striatum. HPLC is used to measure contents of ATP, ADP, and AMP in striatum. Apply bio-chemical lab to analyze the activities of Na+ -K+ -ATPase and Ca2+ -ATPase.
Results:
There appeared a large quantity of positive degenerative neurons in the poisoned rats striatum, but the solution control group did not show similar change. Compared with the solution control group, the ATP contents in the rat striatum of the 2.0 mg/kg and 4.0 mg/kg rotenone groups were significantly decreased (P < 0.01), while the contents of ADP and AMP were relatively raised. With the increase of the poison contents, the activities of Na+ -K -ATPase and Ca2+ -ATPase were inhibited to some degrees. The difference has statistical significance.
Conclusion:
Rotenone could cause the decrease of the ATP content and inhibit activities of Na+ -K+ -ATPase and Ca2+ -ATPase.
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