Related Experiment Video
Updated: Jun 7, 2026
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Published on: February 16, 2014
[Research on utilization of phage display technology to screen chloramine ketone simulated surface point]
Yonghua Xiong1, Xuelan Chen, Xiaoxing Liu
1National Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Objective:
Utilize phage display technology to screen chloramine ketone simulated surface point, providing the basis for establishing drug-free ELISA test system.
Methods:
Use the anti chloramine ketone monoclonal antibody as the ligand, and screen the Ph.D. -7(TM) phage display peptide library to select the chloramine ketone simulated surface point. After 4 rounds of screening, select the phase, which can combine the chloramines ketone monoclonal antibody with different degrees, and further, determine the positive clone with competitive inhibition of the hydrochloric chloramine ketone by the indirect competitive ELISA method.
Results:
It is discovered that all the 10 phages can combine the chloramine ketone monoclonal antibody with different degrees. Use the indirect competitive ELISA method to determine, the phenomenon that 5 positive clones, among them, have competitive inhibition with the hydrochloric chloramine ketone exists. The sequencing results of those 5 positive clones show that 4 different amino acid sequenced are obtained.
Conclusion:
Tentatively judge these 4 phages display the chloramine ketone simulated antigen surface points.
