Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Human T-T cell hybridomas: development and applications.

F E Fox¹, C D Platsoucas

¹Department of Immunology, University of Texas, M.D. Anderson Cancer Center, Houston 77030.

Human Antibodies and Hybridomas

|January 1, 1990

Summary

Human T-T cell hybrids immortalize T cell functions by fusing lymphocytes with tumor cells. A new method enhances hybrid stability and growth without mutagenized cells, improving research applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Phase I study of every 2- or 3-week dosing of ramucirumab, a human immunoglobulin G1 monoclonal antibody targeting the vascular endothelial growth factor receptor-2 in patients with advanced solid tumors.

Annals of oncology : official journal of the European Society for Medical Oncology·2015

Same author

Clinical Experience in Electrically Induced Convulsions.

British medical journal·2010

Same author

"It's crucial they're treated as patients": ethical guidance and empirical evidence regarding treating doctor-patients.

Journal of medical ethics·2009

Same author

Oligoclonal T cells are infiltrating the brains of children with AIDS: sequence analysis reveals high proportions of identical beta-chain T-cell receptor transcripts.

Clinical and experimental immunology·2005

Same author

Pathogenesis of abdominal aortic aneurysms: a multidisciplinary research program supported by the National Heart, Lung, and Blood Institute.

Journal of vascular surgery·2001

Same author

Presence of oligoclonal T cells in cerebrospinal fluid of a child with multiphasic disseminated encephalomyelitis following hepatitis A virus infection.

Clinical and diagnostic laboratory immunology·2001

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

T-T cell hybrids immortalize specific T lymphocyte functions.
Traditional methods used mutagenized tumor cell lines, posing limitations.

Purpose of the Study:

To develop improved methods for generating human T-T cell hybrids.
To enhance the stability and growth characteristics of immortalized T cells.

Main Methods:

Fusion of activated T lymphocytes with human tumor T cell lines.
Selection of hybrids using soft agar colony formation and HLA typing.
Utilizing non-mutagenized tumor cell lines for fusion.

Main Results:

New method generates stable, fast-growing human T-T cell hybrids.
Hybrids successfully produce growth, differentiation, and immunoregulatory factors.
Cell-cell contact-dependent functions are also immortalized.

Conclusions:

The novel T-T cell hybrid generation method offers advantages over older techniques.
This approach enhances T cell immortalization for research and therapeutic development.
Overcomes obstacles associated with previous hybridoma technologies.

Related Experiment Videos