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Updated: Aug 9, 2026

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction
R D'Ovidio1, O A Tanzarella, E Porceddu
1Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Viterbo, Italy.
Abstract:
The polymerase chain reaction (PCR) was used to amplify genomic DNA of several wheat genotypes. The oligonucleotides used as primers were the terminal sequences of a gamma-gliadin gene. The electrophoretic analysis of the PCR products showed specific bands which revealed both inter- and intra-specific genetic polymorphism among the examined genotypes. The technique is proposed as a very simple and efficient alternative to RFLP markers.
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