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Related Concept Videos

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
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DNA Packaging

Overview
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
Chromatin Packaging01:32

Chromatin Packaging

Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...

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Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Classifying DNA assembly protocols for devising cellular architectures.

Xi Wang1, Na Sa, Ping-fang Tian

  • 1College of Life Science and Technology, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Beijing 100029, PR China.

Biotechnology Advances
|November 2, 2010
PubMed
Summary
This summary is machine-generated.

This review covers DNA assembly strategies for synthetic biology, focusing on efficient methods for combining long DNA fragments. It categorizes techniques and proposes a blueprint for creating cell architectures for chemical production.

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Area of Science:

  • Synthetic Biology
  • Molecular Biology
  • Biotechnology

Background:

  • DNA assembly is a cornerstone technique in synthetic biology.
  • Efficient DNA assembly methods are crucial for advancing synthetic biology applications like cellular reprogramming and synthetic cell creation.

Purpose of the Study:

  • To review and categorize typical DNA assembly strategies.
  • To emphasize methods for assembling long and multiple DNA fragments into plasmids or other constructs.
  • To provide a practical, functional overview of DNA assembly applications.

Main Methods:

  • Categorization of DNA assembly strategies into in vivo and in vitro types.
  • Presentation of protocols in a functional and practice-oriented manner.
  • Emphasis on assembling long and multiple DNA fragments.

Main Results:

  • A comprehensive overview of current DNA assembly strategies.
  • Classification of methods based on their in vivo or in vitro nature.
  • A proposed five-step blueprint for designing cell architectures for chemical production.

Conclusions:

  • Efficient DNA assembly is fundamental to achieving advanced synthetic biology goals.
  • The review provides a practical guide to DNA assembly techniques.
  • A novel blueprint is offered for engineering cellular systems for valuable chemical synthesis.