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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

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Published on: February 1, 2018

Large catalase based bioelectrode for biosensor application.

Preety Vatsyayan¹, Sandip Bordoloi, Pranab Goswami

¹Department of Biotechnology, Indian Institute of Technology Guwahati, Assam, India.

Biophysical Chemistry

|November 2, 2010

Summary

This study developed a stable biosensor for hydrogen peroxide (H₂O₂) detection using immobilized catalase (CAT) on a modified electrode. The novel biosensor demonstrates high sensitivity and rapid response, paving the way for practical H₂O₂ monitoring applications.

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Published on: April 18, 2013

Area of Science:

Electrochemistry
Biosensors
Biomaterials

Background:

Direct electrochemistry of enzymes is crucial for biosensor development.
Immobilization strategies are key to enhancing enzyme stability and performance.
Catalase (CAT) is a vital enzyme for H₂O₂ metabolism and detection.

Purpose of the Study:

To develop a stable and sensitive biosensor for hydrogen peroxide (H₂O₂) detection.
To investigate the direct electrochemistry of catalase (CAT) immobilized on a novel electrode matrix.
To evaluate the electrocatalytic activity and kinetic parameters of the developed bioelectrode.

Main Methods:

Immobilization of catalase (CAT) onto a multiwalled carbon nanotubes-Nafion (MWCNT-NF) matrix.
Encapsulation of the immobilized CAT with polyethylenimine (PEI) on a glassy carbon electrode (GCE).
Electrochemical characterization using cyclic voltammetry and chronoamperometry.

Main Results:

The GCE/MWCNT-NF/CAT/PEI bioelectrode exhibited stable and reversible cyclic voltammetric peaks for the Fe(III)/Fe(II) couple.
Polyethylenimine (PEI) enhanced charge transfer and stabilized the bioelectrode.
The biosensor showed a fast response time (~2s), a low detection limit (~1μM), and a wide linear range (10μM to 5mM) for H₂O₂ detection.
High operational and storage stability of the bioelectrode was achieved.

Conclusions:

Direct electrochemistry of large catalase was successfully established on a modified glassy carbon electrode.
The developed bioelectrode demonstrates significant potential for sensitive and stable hydrogen peroxide (H₂O₂) biosensing applications.
The combination of MWCNT-NF matrix and PEI encapsulation offers a promising strategy for robust enzyme-based biosensor design.