Jove
Visualize
Contact Us

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recollections of a Helmstetter Disciple.

Life (Basel, Switzerland)·2023
Same author

Blocking, Bending, and Binding: Regulation of Initiation of Chromosome Replication During the <i>Escherichia coli</i> Cell Cycle by Transcriptional Modulators That Interact With Origin DNA.

Frontiers in microbiology·2021
Same author

Editorial: DNA Replication Origins in Microbial Genomes, Volume 2.

Frontiers in microbiology·2019
Same author

Changing Perspectives on the Role of DnaA-ATP in Orisome Function and Timing Regulation.

Frontiers in microbiology·2019
Same author

Blocking the Trigger: Inhibition of the Initiation of Bacterial Chromosome Replication as an Antimicrobial Strategy.

Antibiotics (Basel, Switzerland)·2019
Same author

Low Affinity DnaA-ATP Recognition Sites in <i>E. coli oriC</i> Make Non-equivalent and Growth Rate-Dependent Contributions to the Regulated Timing of Chromosome Replication.

Frontiers in microbiology·2018
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 7, 2026

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

Regulating DnaA complex assembly: it is time to fill the gaps.

Alan C Leonard1, Julia E Grimwade

  • 1Department of Biological Sciences, 234 Olin Life Sciences, Florida Institute of Technology, 150 W. University Blvd., Melbourne, FL 32901, USA. aleonard@fit.edu

Current Opinion in Microbiology
|November 2, 2010
PubMed
Summary

Bacterial DNA replication timing relies on the initiator protein DnaA interacting with the replication origin (oriC). Its precise regulation involves DnaA binding to specific sites within oriC, ensuring timely chromosome duplication for cell division.

More Related Videos

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
10:32

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Published on: February 3, 2022

Related Experiment Videos

Last Updated: Jun 7, 2026

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
10:32

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Published on: February 3, 2022

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Bacterial chromosome replication initiation is a critical cell division process.
  • The initiator protein DnaA and the replication origin (oriC) are key regulators.
  • Precise timing of replication requires regulated interactions between DnaA-ATP and oriC.

Purpose of the Study:

  • To investigate the role of DnaA recognition sites within oriC in regulating bacterial DNA replication.
  • To understand how DnaA interactions with oriC direct the assembly of pre-replication complexes.
  • To explore the implications of oriC DnaA site variability across bacterial species.

Main Methods:

  • Analysis of DnaA recognition site patterns in oriC sequences.
  • In silico modeling of DnaA-ATP monomer interactions with oriC.
  • Comparative genomics of oriC structures in diverse bacterial taxa.

Main Results:

  • Escherichia coli oriC utilizes high and low affinity DnaA sites for staged assembly of replication initiation complexes.
  • DnaA oligomerization and binding to low affinity sites are crucial for 'filling the gaps' between high affinity sites.
  • Variability in oriC DnaA site patterns suggests diverse strategies for replication control.

Conclusions:

  • The arrangement of DnaA binding sites in oriC dictates the precise timing of DNA replication initiation.
  • Adaptation of oriC structure to DnaA binding patterns allows bacteria to couple replication to specific lifestyles.
  • Understanding these mechanisms provides insights into bacterial cell cycle regulation and evolution.