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Updated: Jun 7, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Counting bacteria on a microfluidic chip.

Yongxin Song¹, Hongpeng Zhang, Chan Hee Chon

¹Department of Marine Engineering, Dalian Maritime University, Dalian 116026, China.

Analytica Chimica Acta

|November 2, 2010

Summary

This summary is machine-generated.

A novel lab-on-a-chip device accurately counts bacteria in solutions using a microfluidic differential Resistive Pulse Sensor (RPS). This automated method enables rapid bacterial concentration determination for various biological applications.

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Area of Science:

Microfluidics
Biosensing
Biotechnology

Background:

Accurate bacterial enumeration is crucial for diagnostics and environmental monitoring.
Existing methods can be time-consuming or require complex instrumentation.
Lab-on-a-chip technologies offer miniaturized and integrated solutions for biological analysis.

Purpose of the Study:

To develop and validate a lab-on-a-chip device for automated bacterial counting.
To utilize a microfluidic differential Resistive Pulse Sensor (RPS) for high-sensitivity detection.
To establish a rapid method for determining bacterial concentrations in aqueous samples.

Main Methods:

Fabrication of a microfluidic chip with a single channel and two differential sensing arms.
Implementation of a Resistive Pulse Sensor (RPS) for detecting bacteria passing through a microchannel.
Correlation of the bacteria flow rate with sample concentration using Pseudomonas aeruginosa.

Main Results:

The microfluidic differential RPS achieved a high signal-to-noise ratio (5-17).
Detected signal amplitudes for Pseudomonas aeruginosa ranged from 0.05 V to 0.17 V.
A linear relationship was observed between bacteria flow rate and sample concentration.

Conclusions:

The developed lab-on-a-chip device provides a simple, automatic, and rapid method for bacterial enumeration.
The microfluidic differential RPS offers a sensitive platform for quantifying bacterial and cell concentrations.
This technology has broad applicability in microbiology and other life science fields.