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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
Published on: November 13, 2017
Sensitive sandwich immunoassay based on single particle mode inductively coupled plasma mass spectrometry detection
1Key Laboratory for Atomic and Molecular Nanosciences of Ministry of Education (MOE), Department of Chemistry, Tsinghua University, Tsinghua Yuan, Beijing 100084, PR China.
Abstract:
A sensitive sandwich type immunoassay has been proposed with the detection by inductively coupled plasma mass spectrometry (ICP-MS) in a single particle mode (time resolved analysis). The signal induced by the flash of ions ((197)Au(+)) due to the ionization of single Au-nanoparticle (Au-NP) label in the plasma torch can be measured by the mass spectrometer. The frequency of the transient signals is proportional to the concentration of Au-NPs labels. Characteristics of the signals obtained from Au-NPs of 20, 45 and 80 nm in diameters were discussed. The analytical figures for the determination of Au-labeled IgG using ICP-MS in conventional integral mode and single particle mode were compared in detail. Rabbit-anti-human IgG was used as a model analyte in the sandwich immunoassay. A detection limit (3 σ) of 0.1 ng mL(-1) was obtained for rabbit-anti-human IgG after immunoreactions, with a linear range of 0.3-10 ng mL(-1) and a RSD of 8.1% (2.0 ng mL(-1)). Finally, the proposed method was successfully applied to spiked rabbit-anti-human IgG samples and rabbit-anti-human serum samples. The method resulted to be a highly sensitive ICP-MS based sandwich type immunoassay.
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