Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Situ Decoding of Vitrimer Topological Evolution: A Non-Invasive, External Stress-Free Approach via Hierarchical CNT Sensing Networks.

ACS applied materials & interfaces·2026
Same author

Clinical Analysis of Comprehensive Pharmacotherapy Combined With Ultrasound-Guided Precise Lesion Resection Plus Primary Microplasty in the Treatment of Nonpuerperal Mastitis.

The breast journal·2026
Same author

Elucidating the structure-property relationships of CL-20/HMX energetic cocrystal materials based on molecular simulation.

The Journal of chemical physics·2026
Same author

Bioanalytical challenges and solutions for immunogenicity evaluation of bispecific antibody therapeutics.

Bioanalysis·2026
Same author

A Chemical-Potential-Driven Self-Mitigation Mechanism during Calendar Aging.

Nano letters·2026
Same author

Iron-sulfur cluster biosynthesis protein OsSUFD is essential for chloroplast function and environmental adaptation in rice.

The Plant journal : for cell and molecular biology·2026

Related Experiment Video

Updated: Jun 7, 2026

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
07:05

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

Published on: November 28, 2017

Clinical immunogenicity specificity assessments: a platform evaluation.

Kun Peng1, Ketevan Siradze, Valerie Quarmby

  • 1Department of BioAnalytical Research & Development, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA. kunp@gene.com

Journal of Pharmaceutical and Biomedical Analysis
|November 2, 2010
PubMed
Summary
This summary is machine-generated.

Assessing immunogenicity in drug development requires specific assays. This study addresses poor specificity in confirmatory assays for antibody therapeutics, offering solutions to improve reliability.

More Related Videos

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice
08:52

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

Published on: February 22, 2019

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
08:48

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

Related Experiment Videos

Last Updated: Jun 7, 2026

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
07:05

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

Published on: November 28, 2017

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice
08:52

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

Published on: February 22, 2019

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
08:48

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

Area of Science:

  • Biopharmaceutical development
  • Drug safety and efficacy evaluation
  • Immunological assays

Background:

  • Immunogenicity assessment is crucial for protein therapeutics safety and efficacy.
  • Regulatory authorities mandate immunogenicity testing.
  • A tiered strategy (screening, confirmation, characterization) is standard.

Purpose of the Study:

  • To address unacceptably poor specificity in the confirmatory step of immunogenicity assays.
  • To investigate challenges encountered with a specific antibody therapeutic (rhuMAb X).
  • To provide solutions for enhancing assay specificity in antibody immunogenicity assessment.

Main Methods:

  • Evaluation of two technology platforms for immunogenicity testing.
  • Assessment of various assay conditions to improve specificity.
  • Utilizing a bridging assay format common in immunogenicity assessments.

Main Results:

  • Observed unacceptably poor specificity in the confirmatory/specificity step for a subset of monoclonal antibodies.
  • Identified challenges with a specific antibody therapeutic (rhuMAb X).
  • Extensive evaluation provided insights into improving assay specificity.

Conclusions:

  • Poor specificity in confirmatory immunogenicity assays is a significant challenge.
  • The findings are relevant to other researchers in the field.
  • Solutions were identified to enhance the specificity of immunogenicity assessments for antibody therapeutics.