Related Experiment Video
Updated: Jun 7, 2026

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings
Published on: May 7, 2020
Acetaminophen inhibits status epilepticus in cultured hippocampal neurons
Laxmikant S Deshpande1, Robert J DeLorenzo
1Department of Neurology, Virginia Commonwealth University, Richmond, Virginia, USA.
Abstract:
Status epilepticus (SE) is a major neurological disorder and SE survivors often develop acquired epilepsy and cognitive deficits. Thus, it is important to stop SE and limit brain damage. However, rapid pharmacoresistance develops to anticonvulsants as seizure duration lengthens. Recently, acetaminophen was reported to increase endocannabinoid levels by its conversion to AM 404. Further, cannabinoids are potent anticonvulsants. Here we investigated whether acetaminophen would block SE-like activity in hippocampal neurons. Exposure of cultured hippocampal neurons to a low Mg2+ medium elicits high-frequency epileptiform discharges that exceed 3 Hz (in-vitro SE). Acetaminophen (500 microM) blocks the SE-like activity. CB1 receptor antagonist SR 141716A (1 microM) blocked this inhibitory effect of acetaminophen on SE, indicating that acetaminophen was mediating its anticonvulsant effects through CB1 receptors.
More Related Videos
06:28High-Quality Seizure-Like Activity from Acute Brain Slices Using a Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System
Published on: September 27, 2024
08:43Long-term Continuous EEG Monitoring in Small Rodent Models of Human Disease Using the Epoch Wireless Transmitter System
Published on: July 21, 2015
Related Concept Videos
Antiepileptic Drugs: GABAergic Pathway Potentiators
The key GABA pathway potentiators used in epilepsy management are as follows.
Benzodiazepines are a well-known class of drugs used for their...
Antiepileptic Drugs: Glutamate Antagonists
Antiepileptic Drugs: Sodium Channel Blockers
Sodium channel blockers modulate ion channels, particularly voltage-gated sodium channels. They block only sodium ion movement.
Among the most commonly prescribed antiepileptic drugs are...
Antiepileptic Drugs: Modulators of Neurotransmitter Release Mediated by SV2A Protein
SV2A is a transmembrane glycoprotein located predominantly in the brain, modulating the release of neurotransmitters for neuronal communication. Both levetiracetam and brivaracetam exhibit a high affinity for...
Epilepsy and Seizures: Overview
Various factors can trigger epilepsy, including genetic factors, brain damage, metabolic causes, and unknown etiology. Diagnosis of epilepsy involves electroencephalography (EEG), which...
Antiepileptic Drugs: Calcium Channel Blockers
Calcium channel blockers exert their antiepileptic effects by targeting T-type calcium channels, which are integral to transmitting nerve signals in the central nervous system. These channels allow the passage of calcium ions, which are vital for neuronal communication. By inhibiting T-type calcium channels, calcium channel blockers effectively reduce the release of neurotransmitters and...