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Overview Of Cell Separation And Isolation

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Updated: Jun 7, 2026

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

Published on: March 27, 2017

A multicellular spheroid formation and extraction chip using removable cell trapping barriers.

Hye-Jin Jin¹, Young-Ho Cho, Jin-Mo Gu

¹Cell Bench Research Center, KAIST, 373-1 Guseong-dong, Yuseong-gu, Daejeon, Republic of Korea 305-701.

|November 2, 2010

Summary

This study introduces a novel multicellular spheroid chip with removable barriers for easy formation and extraction of uniform 3D cancer spheroids. This microfluidic device simplifies spheroid handling for advanced biomedical research.

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Last Updated: Jun 7, 2026

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

Published on: March 27, 2017

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

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Published on: November 7, 2019

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation

Published on: May 12, 2008

Area of Science:

Biomedical Engineering
Microfluidics
3D Cell Culture

Background:

Conventional spheroid formation methods have limitations in uniformity and extraction.
Micro-scale spheroid chips offer better uniformity but face challenges with spheroid extraction due to fixed barriers.

Purpose of the Study:

To design and fabricate a novel multicellular spheroid chip with removable cell trapping barriers.
To enable simple and safe formation and extraction of uniform, small-sized three-dimensional (3D) spheroids.

Main Methods:

A two-layer polydimethylsiloxane (PDMS) spheroid chip was designed and fabricated.
Removable membrane cell trapping barriers were utilized, inflated for spheroid formation and deflated for extraction.
Non-small lung cancer cells (H1650) were cultured in the chip under controlled pressure and incubation conditions.

Main Results:

Uniform 3D spheroids (197.2 ± 11.7 μm diameter) were formed from H1650 cells within 24 hours.
Spheroids were successfully extracted using deflated barriers and outlet ports.
The extracted spheroids maintained high cell viability (80.3 ± 7.7%).

Conclusions:

The developed spheroid chip provides a simple and effective method for generating uniform 3D spheroids.
This technology facilitates the retrieval of spheroids for downstream applications in cell-based research.
The chip is suitable for applications like gene expression analysis and spheroid inoculation in animal models.