A new validated ultra performance liquid chromatographic method for determination of acyclovir
1Department of Pharmaceutics, Jamia Hamdard, Hamdard University, New Delhi, India. dr.sohailhasan@gmail.com
Drug Testing and Analysis
|November 2, 2010
Summary
A new ultra-performance liquid chromatography (UPLC) method was developed to quantify acyclovir. This rapid and sensitive UPLC method ensures accurate analysis of acyclovir in bulk samples.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Ultra-performance liquid chromatography (UPLC) offers significant advancements in chromatographic separations.
- UPLC aims to reduce analysis time while maintaining high separation efficiency.
Purpose of the Study:
- To develop and validate a novel, rapid, and sensitive UPLC method.
- To quantify acyclovir in bulk samples using UV detection.
Main Methods:
- Utilized a Waters Acquity HSS T-3 column (100 × 2.1 mm, 1.8 µm) at 50 °C.
- Employed gradient elution with 1.0% triethyl amine in water and acetonitrile.
- Detection was performed at 254 nm with a flow rate of 0.4 mL/min.
Main Results:
- The developed UPLC method demonstrated selectivity, linearity, accuracy, and precision.
- The method adhered to International Council for Harmonisation (ICH) guidelines.
- Achieved a detection limit of 3 ng/mL and a quantitation limit of 8 ng/mL for acyclovir.
Conclusions:
- A validated, rapid, and sensitive UPLC-UV method for acyclovir quantification in bulk samples was successfully established.
- The method meets stringent analytical requirements for pharmaceutical analysis.
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