Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Properties of Enantiomers and Optical Activity02:24

Properties of Enantiomers and Optical Activity

It is essential to understand the difference between chiral and achiral interactions and the implications thereof in optical activity and their applications. Just as our feet, which are chiral, interact uniquely with chiral objects, such as a pair of shoes, but identically with achiral socks, enantiomers of a molecule exhibit different properties only when they interact with other chiral media. An example of a significant implication from this facet is the phenomenon known as optical activity,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unwarranted variation in total B12 test thresholds for the diagnosis of vitamin B12 deficiency across the UK and the validation of a method-specific total B12 reference interval for the Beckman Coulter DxI platform to support implementation of NICE NG239.

Clinical chemistry and laboratory medicine·2026
Same author

Shift happens again: a call to action for EQA providers to rise to the post market surveillance challenge.

Clinical chemistry and laboratory medicine·2026
Same author

Clinical quality in UK laboratory medicine: current status and what the wider laboratory community can learn.

Clinical chemistry and laboratory medicine·2026
Same author

Total Serum Calcium Spike-Recovery Experiments to Investigate Bones of Contention in Clinical Laboratories.

The journal of applied laboratory medicine·2026
Same author

Sustained effect of Scotland's HIV pre-exposure prophylaxis programme on transmission among gay and bisexual men who have sex with men: Population-based retrospective cohort study.

HIV medicine·2026
Same author

Stigma and depression among heterosexual men and women living with HIV in the UK: Investigating a conceptual framework.

PloS one·2026
Same journal

AQbD-Guided Chiral HPLC for Enantiomeric Impurity Analysis of Suzetrigine.

Chirality·2026
Same journal

Recent Progress in the Total Synthesis and Biosynthesis of Arborisidine.

Chirality·2026
Same journal

Advances in Chiral Separation Techniques for Calcium Channel Blockers: Analytical Strategies and Future Perspectives in Antihypertensive Drug Development (2015-2025).

Chirality·2026
Same journal

Practical Enantioselective Approach to 3-Amino-2-Hydroxy Acids and Application to the Synthesis of Natural Products.

Chirality·2026
Same journal

Chiral (Stereoselective) Drugs, Asymmetric Synthesis, and Racemic Resolution Methods.

Chirality·2026
Same journal

Capillary Chiral Separation of Agrochemicals: Complementary Insights From Molecular Modeling and NMR.

Chirality·2026
See all related articles

Related Experiment Video

Updated: Jun 7, 2026

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

Capillary circular dichroism.

Daniel E Waldron1, Rachel Marrington, Marcus C Grant

  • 1Department of Chemistry, University of Warwick, Coventry, United Kingdom.

Chirality
|November 2, 2010
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel circular dichroism (CD) sample holder using quartz capillaries, drastically reducing sample volume requirements for biological molecule analysis. This innovation enables structural studies with minimal sample, making CD spectroscopy more accessible.

More Related Videos

A Micropatterning Assay for Measuring Cell Chirality
08:07

A Micropatterning Assay for Measuring Cell Chirality

Published on: March 11, 2022

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Related Experiment Videos

Last Updated: Jun 7, 2026

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

A Micropatterning Assay for Measuring Cell Chirality
08:07

A Micropatterning Assay for Measuring Cell Chirality

Published on: March 11, 2022

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Area of Science:

  • Biophysical Chemistry
  • Spectroscopy
  • Structural Biology

Background:

  • Circular dichroism (CD) spectroscopy is crucial for analyzing biological molecule structures in solution.
  • Conventional CD measurements demand large sample volumes (100-300 μL of 0.1 mg/ml protein), limiting its application.
  • High sample requirements hinder the widespread use of CD for structural elucidation.

Purpose of the Study:

  • To design and validate a new CD sample holder that significantly reduces sample volume requirements.
  • To overcome the limitation of high sample consumption in traditional CD spectroscopy.
  • To enhance the accessibility and efficiency of structural analysis for biological molecules using CD.

Main Methods:

  • Development of a novel CD sample holder utilizing extruded quartz capillaries.
  • Employing capillary action to hold samples within the quartz capillary's internal diameter.
  • Investigating optical configurations, including focusing the spectrophotometer's light beam with a biconvex lens and utilizing the capillary's focusing effect.
  • Acquiring data down to 180 nm using a Jasco J-815 spectrophotometer.

Main Results:

  • The new sample holder reduces sample requirements by two orders of magnitude, needing less than 3 μL.
  • Extruded quartz capillaries exhibit minimal birefringence while effectively transmitting UV circularly polarized light.
  • Reliable CD spectral data were obtained down to 180 nm.
  • System performance was validated using various samples including a coordination complex, concanavalin A, lysozyme, and progesterone.

Conclusions:

  • The developed quartz capillary-based CD sample holder significantly lowers sample volume needs for CD spectroscopy.
  • This innovation makes CD analysis more feasible for precious or limited biological samples.
  • The system provides high-quality structural data at low wavelengths, expanding the utility of CD spectroscopy.