Evaluation of instrumental errors built in circular dichroism spectrometers
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Updated: Jun 7, 2026

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
Published on: August 18, 2017
Ettore Castiglioni1, Paolo Albertini, Sergio Abbate
1JASCO Corporation, Ishikawa-cho, Hachioji-Shi, Tokyo 192-8537, Japan. ettore.castiglioni@jasco-europe.com
Abstract:
Because of the increased use of circular dichroism (CD) spectroscopy as a routine technique by nonspecialists to determine the conformational/configurational properties of biomolecules, we have decided to present here some criteria to accurately check the ordinate scale calibration of a CD spectrometer particularly in the critical low-wavelength UV region, to understand, and correct, where possible, the potential limitations coming from the hardware. We also analyze some wavelength calibration methods, and some standards for the CD-scale calibration, and we discuss the critical characteristics of current instrumentation affecting measurements. The example of the bovine catalase CD spectrum is considered.
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