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Uterus-derived growth substances: possible implications for human uterus physiology and pathophysiology (review). The
1Department of Obstetrics and Gynecology, St-François d'Assise Hospital, Laval University Quebec, Canada.
Abstract:
The presence of mitogens and inhibitors in myometrial, endometrial and leiomyoma extracts was documented. Growth substances of human uterus extracts were sensitive to tryptic digestion and were acid stable. Mitogens and inhibitors were retained and could be eluted from cartridges of octadecylsilyl-silica by 80% acetonitrile. Reverse-phase high performance liquid chromatography (r-HPLC) could separate mitogens from inhibitors. In addition, r-HPLC profiles revealed eluates of myometrial and leiomyoma extracts containing mitogens with preferential activity for either osteoblasts or myoblast-like cells, respectively. The presence of mitogens and inhibitors in human uterine extracts suggests a possible involvement of these growth substances in the regulation of various physiological and pathophysiological processes of human uterus.