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Updated: Jun 7, 2026
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
Published on: June 12, 2018
[Detection of telomerase activity in the blood of patients with lung cancer]
1Shanghai Institute of Thoracic Tumors, Shanghai Chest Hospital, Shanghai 200030, P.R.China.
Background:
To study the telomerase activity in blood cancer cells by TRAP for monitoring tumor metastasis in blood.
Methods:
Twenty-five patients with lung cancer surgically treated and 35 patients before chemotherapy were determined for telomerase activity of cancer cells in the blood by TRAP, and 30 patients with non-tumor diseases as control.
Results:
In the operative group,13 patients(52%) showed telomerase activity in pulmonary artery blood during operation, which was much higher than that of peripheral blood before operation (24%,P<0.05). Telomerase positive rate was significantly higher in stage III to IV (64%) than that in stage I to II (30%,P<0.05).In the peripheral blood of patients with non-tumor diseases, telomerase was negative.
Conclusions:
Telomerase activity may be an indicator for detecting lung cancer cells in peripheral blood as well as tumor metastasis and relapse.
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