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Related Concept Videos

Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Positron Emission Tomography01:29

Positron Emission Tomography

Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body being...
Imaging Studies I: CT and MRI01:14

Imaging Studies I: CT and MRI

Introduction: MRI and CT scans are crucial advancements in medical imaging techniques, playing a vital role in diagnosing conditions related to the gastrointestinal (GI) system. Each scan serves distinct purposes, targets specific areas, and requires unique nursing duties.
Description of the Procedures
Computed Tomography (CT) scan:
Computed Tomography (CT) scans use X-ray technology to generate detailed images of bones, organs, and tissues. During the scan, the patient lies on a moving table...
Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET

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Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

Array tomography: production of arrays.

Kristina D Micheva, Nancy O'Rourke, Brad Busse

    Cold Spring Harbor Protocols
    |November 3, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Array tomography uses serial sectioning for 3D microscopy. This technique enables detailed visualization and quantification of tissue structures and molecular architecture, offering new insights into biological complexity.

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    Area of Science:

    • Microscopy
    • Cell Biology
    • Histology

    Background:

    • Array tomography is a volumetric microscopy technique.
    • It relies on physical serial sectioning of embedded tissues.
    • This method allows for 3D reconstruction and analysis.

    Purpose of the Study:

    • To describe a protocol for array tomography.
    • To detail the sectioning and mounting of serial arrays.
    • To enable visualization of tissue structure and molecular architecture.

    Main Methods:

    • Physical serial sectioning of plastic-embedded tissue using an ultramicrotome.
    • Bonding ultrathin sections into an ordered array on a coverslip.
    • Staining, imaging, and computational reconstruction into 3D volumes.

    Main Results:

    • High-quality rapid staining and imaging due to minimal section thickness.
    • Reliable and convenient section handling, staining, and automated imaging.
    • Enables repeated imaging cycles and multi-modal imaging (fluorescence, electron microscopy).

    Conclusions:

    • Array tomography provides unprecedented visualization and quantification of tissue and molecular architecture.
    • Careful tissue preparation is crucial for successful implementation.
    • The protocol requires familiarity with ultramicrotome sectioning techniques.