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Neutron scattering and protein dynamics

Hans Frauenfelder1, Ferenc Mezei

  • 1Los Alamos National Laboratory, USA. frauenfelder@lanl.gov

Acta Crystallographica. Section D, Biological Crystallography
|November 3, 2010
PubMed

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...