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Related Experiment Videos

The study on transgenic mice.

Y X Shi1, X D Ji, G S Li

  • 1Institute of Developmental Biology, Chinese Academy of Sciences, Beijing.

Chinese Journal of Biotechnology
|January 1, 1990
PubMed
Summary
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Transgenic mice carrying a fused gene for human growth hormone (hGH) exhibited larger size and hGH production. These traits were heritable, though body weight decreased in subsequent generations.

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • The metallothionein-1 promoter is a well-characterized inducible promoter in mice.
  • Human growth hormone (hGH) plays a crucial role in postnatal growth and development.
  • Genetic engineering offers a pathway to study gene function and expression in vivo.

Purpose of the Study:

  • To create transgenic mice expressing human growth hormone (hGH) under the control of the mouse metallothionein-1 (MT) promoter.
  • To investigate the inducibility and heritability of the MT-hGH fusion gene.
  • To assess the physiological effects of the introduced gene on mouse growth and development.

Main Methods:

  • Construction of a fusion gene linking the hGH structural gene to the MT-1 promoter.

Related Experiment Videos

  • Microinjection of the MT-hGH fusion gene into pronuclei of fertilized mouse eggs.
  • Implantation of engineered embryos into pseudo-pregnant foster mothers.
  • Genomic DNA analysis (dot hybridization, Southern blotting) to confirm gene integration.
  • RNA analysis (Northern blotting) to confirm gene transcription.
  • Monitoring of body weight and growth parameters in transgenic and control mice.

    • Main Results:

    • Eighteen out of forty-three born mice successfully integrated the MT-hGH fusion gene.
    • Transgenic mice showed increased body size compared to wild-type controls.
    • hGH synthesis was inducible by zinc administration, consistent with MT promoter activity.
    • The hGH gene was transcribed into mRNA in the livers of transgenic mice.
    • The integrated gene was stably inherited across multiple generations.
    • A decrease in body weight was observed in subsequent generations of transgenic mice.

    Conclusions:

    • The MT-hGH fusion gene is functional in transgenic mice, leading to hGH expression and altered growth.
    • The expression of the fusion gene is inducible and heritable.
    • While initial growth is enhanced, long-term effects on body weight in progeny warrant further investigation.
    • This model provides a valuable tool for studying hGH regulation and its effects on mammalian development.