Related Experiment Video
Updated: Jun 7, 2026
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Isolation of two bioactive diterpenic acids from Copaifera glycycarpa oleoresin by high-speed counter-current
P A De Souza1, L P Rangel, S S Oigman
1Universidade Federal do Rio de Janeiro, Departamento de Química Orgânica, Rio de Janeiro, RJ, Brazil.
Introduction:
Phytochemical and biological studies carried out on Copaifera species showed that their oleoresins and isolated compounds have various biological activities.
Objective:
The aims of this work were (i) to analyse the Copaifera oleoresin by gas chromatography-mass spectrometry, (ii) to isolate the diterpenic acids from this oleoresin by high-speed countercurrent chromatography (HSCCC) and (iii) to determine the rhodamine 6G Pdr5p activity of these acids.
Methodology:
HSCCC was used for the preparative separation of the diterpenes. Spectroscopic methods were used to establish their identity.
Results:
The gas chromatogram of the oleoresin showed approximately 30 compounds. The two major ones, kaur-16-en-18-oic and polyalthic acids, were isolated in high purity. Kaur-16-en-18-oic acid exhibited the highest rodomine 6G Pdr5p activity among the tested compounds.
Conclusion:
HSCCC was shown to be a quick and effective tool in the isolation and purification of diterpenes from Copaifera oleoresin. This is the first report on the use of HSCCC for the fractionation of an oleoresin from Copaifera and the isolation of diterpenes therein.
More Related Videos
11:44Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
Published on: January 19, 2022
08:56Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
