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Related Concept Videos

Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
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Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
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Related Experiment Video

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Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
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Ultrastructural expression of P-selectin on surface activated platelets.

G Escolar1, G H Rao, H K Nieuwenhuis

  • 1Servicio de Hemoterapia y Hemostasia, Hospilat Clinic, University of Barcelona, The Netherlands.

Platelets
|November 4, 2010
PubMed
Summary

P-selectin expression on platelets (P1) is released via surface-activation, not solely granule transport. Its movement from the central zone outward suggests a pathway through the open canalicular system.

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Last Updated: Jun 7, 2026

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Area of Science:

  • Hematology
  • Cell Biology
  • Biochemistry

Background:

  • P-selectin is a glycoprotein found in platelet alpha granules.
  • Its surface expression upon activation is crucial for platelet function.
  • The exact mechanism of P-selectin surface expression remains unclear.

Purpose of the Study:

  • To investigate the mechanism of P-selectin surface expression in activated platelets.
  • To determine if P-selectin is transported from granule membranes or released by surface-activation.
  • To elucidate the spatial and temporal distribution of P-selectin during platelet spreading.

Main Methods:

  • Washed platelets were allowed to interact with grids for varying durations (5-20 min).
  • Platelets were fixed and exposed to a monoclonal antibody against P-selectin.
  • Immunogold labeling with anti-mouse IgG coupled to 10 nm gold particles was used.
  • Electron microscopy was employed to examine the distribution of gold probes.

Main Results:

  • Discoid platelets showed no P-selectin expression.
  • Early dendritic platelets exhibited P-selectin near the central zone.
  • Late dendritic and fully spread platelets displayed P-selectin distribution, concentrated peripherally.
  • P-selectin probes were evenly distributed on fully spread platelets, with higher concentration at the margin.

Conclusions:

  • P-selectin is released from activated platelets (SfA P1).
  • Initial central zone expression suggests transport via the open canalicular system.
  • The centrifugal movement of P-selectin contrasts with receptor-ligand complex translocation.