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Related Concept Videos

Vitamins01:30

Vitamins

Vitamins, derived from the Latin word for life, are essential organic substances required in small quantities for optimal growth and overall well-being. Unlike other organic nutrients, vitamins don't act as sources of energy or building materials but rather facilitate these nutrients' utilization by the body. Vitamins are predominantly coenzymes, assisting enzymes in specific chemical actions, like the oxidation of glucose for energy involving B vitamins. Most vitamins are not produced in our...
Disorders of Hemostasis01:24

Disorders of Hemostasis

Hemostasis, the process that stops bleeding after a blood vessel injury, is crucial for maintaining the integrity of the circulatory system. However, disorders of hemostasis can disrupt this delicate balance, leading to either excessive clotting or bleeding. These disorders can be broadly classified into thromboembolic disorders and bleeding disorders.
Thromboembolic Disorders
Two factors primarily cause thromboembolic conditions.
Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
Role of Vitamins in Maintaining Bone Health01:25

Role of Vitamins in Maintaining Bone Health

The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...

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Related Experiment Video

Updated: Jun 7, 2026

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

Platelet dysfunction in nutritional vitamin b(12) deficiency.

K Ghosh1, V Krishna, D Mohanty

  • 1Haematology Laboratory, Farwaniya Hospital.

Platelets
|November 4, 2010
PubMed
Summary

Nutritional vitamin B12 deficiency commonly impairs platelet aggregation, affecting responses to various agonists. However, this platelet dysfunction does not consistently predict clinical bleeding tendencies in patients.

Area of Science:

  • Hematology
  • Nutritional Biochemistry
  • Clinical Medicine

Background:

  • Vitamin B12 is crucial for DNA synthesis and cellular metabolism, including platelet production and function.
  • Nutritional deficiencies can impact hemostasis, but the specific effects of vitamin B12 deficiency on platelet aggregation are not fully elucidated.
  • Coexisting iron deficiency is common and may influence hematological parameters.

Purpose of the Study:

  • To investigate the effect of nutritional vitamin B12 deficiency on platelet aggregation.
  • To assess the correlation between impaired platelet aggregation and clinical bleeding manifestations.
  • To examine the influence of coexisting iron deficiency on these parameters.

Main Methods:

  • Platelet-rich plasma was obtained from 17 patients with nutritional vitamin B12 deficiency.

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Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
07:06

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

Related Experiment Videos

Last Updated: Jun 7, 2026

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
07:06

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

  • Platelet aggregation was measured using four different agonists: ADP, collagen, epinephrine, and ristocetin.
  • Clinical bleeding history was recorded, and patients were categorized based on deficiency status (B12 alone or B12 and iron).

    • Main Results:

    • Impaired platelet aggregation was observed in a significant number of patients across different agonists.
    • Specifically, 4/17 patients showed impaired aggregation to ADP, 8/17 to collagen, 10/17 to epinephrine, and 6/17 to ristocetin.
    • Bleeding manifestations occurred in 5 patients, but three of them had normal platelet aggregation, indicating a weak correlation.

    Conclusions:

    • Nutritional vitamin B12 deficiency is frequently associated with impaired platelet aggregation.
    • The degree of platelet dysfunction does not reliably correlate with the presence or severity of clinical bleeding.
    • Further research is needed to understand the complex interplay between vitamin B12, platelet function, and bleeding risk.