Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Formation of the Platelet Plug

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

Structure and Function of Platelets

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

Nitric Oxide Signaling Pathway

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...

Feedback Inhibition

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Messenger RNA encoding constitutively active Toll-like receptor 4 enhances effector functions of human T cells.

Clinical and experimental immunology·2015

Same author

42 kDa Protein as a Substrate for Protein Phosphatase (s) in Intact Human Blood Platelets.

Platelets·2010

Same author

Protein Kinase C of Human Platelets: Resolution of Ca(2+)-dependent and Independent Forms by Measuring Endogenous Phosphorylation.

Platelets·2010

Same author

Original article: low regulatory volume decrease rate in platelets from ischemic patients: a possible role for hepoxilin a(3) in thrombogenicity.

Platelets·2010

Same author

Targeting tumor-associated antigens to the MHC class I presentation pathway.

Endocrine, metabolic & immune disorders drug targets·2007

Same author

A comparison of videotape and audiotape assessment of patient-centredness in family physicians' consultations.

Patient education and counseling·2001

Same journal

Hexokinase controls platelet activation and hemostasis.

Platelets·2026

Same journal

Chemiluminescence resonance energy transfer-based method to investigate the platelet surface molecule in acute myocardial infarction.

Platelets·2026

Same journal

Nanobodies to GPVI as alternative reagents for platelet spreading.

Platelets·2026

Same journal

Neural network reveals platelet age from fluorescence microscopy images.

Platelets·2026

Same journal

Sixty years of research into ancestry differences in platelet function.

Platelets·2026

Same journal

Platelet-rich plasma concentrations regulate MSCs osteogenesis via MAPK/PI3K-AKT pathways to mitigate inflammatory bone loss.

Platelets·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 7, 2026

Alternative Methods for the Detection of Superoxide Anion Generation in Platelets

Alternative Methods for the Detection of Superoxide Anion Generation in Platelets

Published on: March 29, 2024

Lipoxygenase product controls the regulatory volume decrease of human platelets.

A Margalit¹, A A Livne

¹Department of life Sciences (Biology), Ben-Gurion University, Beer-Sheva, Israel, 84105.

|November 4, 2010

Summary

This summary is machine-generated.

Platelet regulatory volume decrease (RVD) is controlled by a lipoxygenase product, which selectively regulates potassium (K+) permeability. This finding was supported by experiments using lipoxygenase inhibitors and products.

More Related Videos

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Related Experiment Videos

Last Updated: Jun 7, 2026

Alternative Methods for the Detection of Superoxide Anion Generation in Platelets

Alternative Methods for the Detection of Superoxide Anion Generation in Platelets

Published on: March 29, 2024

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Area of Science:

Biochemistry
Cell Physiology
Hematology

Background:

Blood platelets exhibit regulatory volume decrease (RVD) when exposed to hypotonic environments.
RVD is typically mediated by increased conductive permeability of potassium (K+) and chloride (Cl-) ions.

Purpose of the Study:

To investigate the role of lipoxygenase products in platelet RVD.
To determine if lipoxygenase products selectively regulate ion permeability during RVD.

Main Methods:

Inhibition of RVD using various lipoxygenase inhibitors (NDGA, BWA4C, CGS 8515, 5,8,11,14-eicosatetraynoic acid) and assessment of cyclooxygenase inhibitors (aspirin, indomethacin).
Analysis of an eluate from RVD-inducing platelets for RVD-restoring activity.
Testing the effect of known lipoxygenase products (12-HPETE, 12-HETE, LTD4) on RVD in inhibitor-treated platelets.
Examining the effect of NDGA on hypotonic swelling in gramicidin-treated platelets to assess Cl(-) permeability.

Main Results:

Lipoxygenase inhibitors significantly inhibited RVD, while cyclooxygenase inhibitors had no effect.
An unstable eluate from RVD platelets restored RVD in inhibitor-treated platelets, and albumin enhanced RVD.
Specific lipoxygenase products restored RVD in NDGA-treated platelets.
Potassium (K+) permeability regulation, not chloride (Cl-), appears to be selectively controlled by lipoxygenase products.

Conclusions:

A lipoxygenase product plays a crucial role in regulating platelet RVD.
This lipoxygenase product appears to selectively open potassium (K+) channels in platelets.
The findings suggest a mechanism analogous to lipoxygenase product effects in cardiac atrial cells and neurons.