Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Common calcaneal tendon repair in cats: outcomes and complications associated with different postoperative tarsocrural joint immobilisation methods.

The Journal of small animal practice·2025
Same author

Diaphragmotomy to aid exposure during hepatobiliary surgery: a multi-centre retrospective review of 31 dogs.

The Journal of small animal practice·2020
Same author

Surgical management of impalement injuries to the trunk of dogs: a multicentre retrospective study.

The Journal of small animal practice·2017
Same author

Isoform specific differences in phospholipase C beta 1 expression in the prefrontal cortex in schizophrenia and suicide.

NPJ schizophrenia·2017
Same author

Evidence for impaired glucose metabolism in the striatum, obtained postmortem, from some subjects with schizophrenia.

Translational psychiatry·2016
Same author

Fatty acid composition of the postmortem corpus callosum of patients with schizophrenia, bipolar disorder, or major depressive disorder.

European psychiatry : the journal of the Association of European Psychiatrists·2016
Same journal

Hexokinase controls platelet activation and hemostasis.

Platelets·2026
Same journal

Chemiluminescence resonance energy transfer-based method to investigate the platelet surface molecule in acute myocardial infarction.

Platelets·2026
Same journal

Nanobodies to GPVI as alternative reagents for platelet spreading.

Platelets·2026
Same journal

Neural network reveals platelet age from fluorescence microscopy images.

Platelets·2026
Same journal

Sixty years of research into ancestry differences in platelet function.

Platelets·2026
Same journal

Platelet-rich plasma concentrations regulate MSCs osteogenesis via MAPK/PI3K-AKT pathways to mitigate inflammatory bone loss.

Platelets·2026
See all related articles

Related Experiment Video

Updated: Jun 7, 2026

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
05:49

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

Phorbol esters increase [(3)h]-dopamine uptake by human platelets.

B Dean1, D L Copolov

  • 1Neurochemistry Unit, The Mental Health Research Institute of Victoria, Private Mail Bag No. 3, Parkville, Victoria 3052, and The NH and MRC Schizophrenia Unit, Royal Park Hospital, Parkville, Victoria, 3052, Australia.

Platelets
|November 4, 2010
PubMed
Summary
This summary is machine-generated.

Phorbol esters enhance dopamine uptake in human platelets by activating protein kinases. This discovery sheds light on factors influencing dopamine transport and potential therapeutic targets.

More Related Videos

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
08:04

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Related Experiment Videos

Last Updated: Jun 7, 2026

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
05:49

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
08:04

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pharmacology

Background:

  • Dopamine uptake by platelets is an energy-dependent process.
  • Altered dopamine uptake in platelets is observed in various disease states.
  • Factors regulating platelet dopamine uptake remain largely unknown.

Purpose of the Study:

  • To investigate the effect of phorbol esters on dopamine uptake by human platelets.
  • To determine if protein kinases play a role in modulating platelet dopamine uptake.

Main Methods:

  • Human platelets were incubated with various phorbol esters.
  • [(3)H]-dopamine uptake was measured in the presence of phorbol esters.
  • The effect of a protein kinase inhibitor (Staurosporin) was examined.

Main Results:

  • Phorbol 12,13-dibutyrate and phorbol 12,13-didecanoate significantly increased [(3)H]-dopamine uptake in a dose-dependent manner.
  • 4α phorbol 12,13-didecanoate, which does not affect protein kinase, did not enhance dopamine uptake.
  • Staurosporin reversed the phorbol ester-induced increase in dopamine uptake.

Conclusions:

  • Protein kinases modulate dopamine uptake by human platelets.
  • Phorbol esters, via protein kinase activation, can enhance platelet dopamine uptake.
  • These findings suggest a novel regulatory mechanism for dopamine transport in platelets.