Related Experiment Video
Updated: Jun 7, 2026
10:38
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
Published on: October 8, 2013
Ozone induces inflammation in bronchial epithelial cells
Hong Song1, Weipin Tan, Xuchao Zhang
1Department of Preventive Medicine, School of Public Health, Sun Yat-Sen University, Guangzhou, P.R. China. songhong@mail.sysu.edu.cn
Summary
Ozone exposure increases inflammatory markers and oxidative stress in airway epithelial cells. This may contribute to airway inflammation, especially in children with asthma.
Area of Science:
- Environmental Health
- Pulmonology
- Toxicology
Background:
- Ozone is a key vehicle exhaust pollutant linked to childhood asthma.
- Mechanisms of ozone's effect on airway epithelium and inflammation are not fully understood.
Purpose of the Study:
- To investigate ozone's impact on human bronchial epithelial cells.
- To determine ozone's role in airway inflammation, particularly in asthmatic individuals.
Main Methods:
- Human bronchial epithelial cells exposed to ozone (0.16 mg/m³).
- Measured Interleukin-1 (IL-1) and IL-6 secretion via ELISA.
- Assessed intracellular malondialdehyde (MDA) for oxidative stress.
- Evaluated conditioned media's effect on T lymphocytes from asthmatic and healthy subjects using ELISA and RT-PCR.
Main Results:
- Ozone exposure increased IL-1 and IL-6 production in a time-dependent manner.
- Ozone significantly elevated MDA levels, indicating oxidative stress.
- Ozone-exposed cell media stimulated IL-1 mRNA in T lymphocytes from asthmatic patients but not healthy subjects.
Conclusions:
- Low-level, short-term ozone exposure induces pro-inflammatory markers and oxidative stress in epithelial cells.
- These cellular changes may exacerbate airway inflammation in asthmatic children.
Related Concept Videos
01:24Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features
Chronic bronchitis is a key phenotype of chronic obstructive pulmonary disease (COPD), characterized by airway-centered inflammation and mucus overproduction. It develops from long-term exposure to harmful particles or gases, most commonly cigarette smoke, which triggers a persistent inflammatory response.Cellular and Structural ChangesInflammation initially affects the large bronchi and later the smaller airways, with infiltration by immune cells, including neutrophils, macrophages, and...
01:20Chronic Obstructive Pulmonary Disease-II: Pathophysiology
Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
Chronic Inflammation
01:28Asthma I: Introduction
Asthma is a chronic inflammatory disorder of the airways characterized by variable airflow obstruction and heightened bronchial responsiveness to a wide range of triggers. The underlying inflammation leads to airway swelling, mucus hypersecretion, and smooth muscle constriction, all of which narrow the airway lumen and impede airflow. Clinically, asthma presents with recurrent episodes of wheezing, shortness of breath, chest tightness, and coughing, symptoms that typically vary in intensity and...
01:26Asthma-II: Pathophysiology and Classification
Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
01:29Asthma-I: Introduction
Asthma is a chronic respiratory ailment that requires careful management due to its varying symptoms and influencing factors. It is characterized by airway inflammation, bronchial hyperresponsiveness, and reversible airflow obstruction, leading to symptoms like wheezing, shortness of breath, chest tightness, and coughing. The symptom frequency and intensity may vary considerably over time. It is also linked to immune system responses to allergens and irritants, highlighting the complex...
01:46Oxidative Cleavage of Alkenes: Ozonolysis
In ozonolysis, ozone is used to cleave a carbon–carbon double bond to form aldehydes and ketones, or carboxylic acids, depending on the work-up.
Ozone is a symmetrical bent molecule stabilized by a resonance structure.
Ozone is a symmetrical bent molecule stabilized by a resonance structure.