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Updated: Jun 7, 2026

Magnetic Tweezers for the Measurement of Twist and Torque
Published on: May 19, 2014
Effect of spontaneous twist on DNA minicircles
Shlomi Medalion1, David A Kessler, Yitzhak Rabin
1Department of Physics, Bar-Ilan University, Ramat-Gan, Israel. shlomed.uni@gmail.com
Abstract:
Monte Carlo simulations are used to study the effect of spontaneous (intrinsic) twist on the conformation of topologically equilibrated minicircles of dsDNA. The twist, writhe, and radius of gyration distributions and their moments are calculated for different spontaneous twist angles and DNA lengths. The average writhe and twist deviate in an oscillatory fashion (with the period of the double helix) from their spontaneous values, as one spans the range between two neighboring integer values of intrinsic twist. Such deviations vanish in the limit of long DNA plasmids.
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