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Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
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Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter
Toshitsugu Sato1, Kumiko Okawa, Tatsuya Hirano
1Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate 024-0003, Japan. tosisato@mail.kitami-it.ac.jp
Journal of Bioscience and Bioengineering
|November 4, 2010
Abstract:
Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation.
