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Transport Across the Golgi01:26

Transport Across the Golgi

While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
Golgi Apparatus01:49

Golgi Apparatus

As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.The Golgi apparatus is a major sorting and dispatch station for the products of the ER. Newly arriving vesicles enter...
Golgi Apparatus01:09

Golgi Apparatus

Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
Golgi Apparatus01:09

Golgi Apparatus

Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
Golgi Matrix Proteins01:12

Golgi Matrix Proteins

Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...

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Related Experiment Video

Updated: Jun 7, 2026

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
13:08

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Published on: August 10, 2017

How the Golgi works: a cisternal progenitor model.

Suzanne R Pfeffer1

  • 1Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307, USA. pfeffer@stanford.edu

Proceedings of the National Academy of Sciences of the United States of America
|November 4, 2010
PubMed
Summary

The Golgi complex, crucial for protein processing in eukaryotic cells, has its assembly and protein transport mechanisms explored. A new model suggests cargo moves through a stacked Golgi via fusion and fission of stable compartments.

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4D Microscopy of Yeast
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Related Experiment Videos

Last Updated: Jun 7, 2026

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
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Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Published on: August 10, 2017

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
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4D Microscopy of Yeast
12:00

4D Microscopy of Yeast

Published on: April 28, 2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The Golgi complex is a vital organelle in eukaryotic cells, central to the secretory pathway.
  • It processes over 30% of human proteins, but its assembly and protein transport remain incompletely understood.

Purpose of the Study:

  • To elucidate the assembly process of the Golgi complex.
  • To understand the mechanisms of protein transport through the Golgi apparatus.

Main Methods:

  • Review of recent advances in protein transport and endocytic pathways.
  • Development of a theoretical model for Golgi function and cargo transport.

Main Results:

  • A model is proposed for protein transport through a stacked Golgi.
  • This model involves continuous fusion and fission of stable, similar subcompartments.

Conclusions:

  • The proposed model offers insights into Golgi complex assembly and ordered protein transport.
  • It suggests a mechanism for Golgi growth from a stable progenitor.