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Related Experiment Video

Updated: Jun 7, 2026

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion
05:58

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

Published on: July 4, 2018

Exploring endocrine GH pattern in mice using rank plot analysis and random blood samples.

Jie Xu1, Amaury Jean-Marie Bekaert, Joëlle Dupont

  • 1INSERM U938, Hôpital Saint-Antoine, Bâtiment Kourilsky, 184 rue du Faubourg Saint-Antoine, F-75012 Paris, France.

The Journal of Endocrinology
|November 4, 2010
PubMed
Summary
This summary is machine-generated.

We developed a new method to study growth hormone (GH) secretion patterns in mice. This approach allows for easier analysis of GH levels, revealing important insights into its regulation and function.

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Related Experiment Videos

Last Updated: Jun 7, 2026

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion
05:58

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Published on: July 4, 2018

Remote Neuronal Activation Coupled with Automated Blood Sampling to Induce and Measure Circulating Luteinizing Hormone in Mice
08:56

Remote Neuronal Activation Coupled with Automated Blood Sampling to Induce and Measure Circulating Luteinizing Hormone in Mice

Published on: August 25, 2023

In vivo Characterization of Endocrine Disrupting Chemical Effects via Thyroid Hormone Action Indicator Mouse
04:14

In vivo Characterization of Endocrine Disrupting Chemical Effects via Thyroid Hormone Action Indicator Mouse

Published on: October 6, 2023

Area of Science:

  • Endocrinology
  • Physiology
  • Animal Models

Background:

  • Growth hormone (GH) is crucial for vertebrate development, growth, metabolism, and aging.
  • Studying GH secretion pulsatility in mice is challenging due to technical limitations with sampling.
  • Altered GH signaling in mouse models is vital for understanding somatotropic pathophysiology.

Purpose of the Study:

  • To develop a practical method for analyzing endogenous, physiological GH secretion patterns in mice from spot samples.
  • To investigate the characteristics of GH secretion, including circadian and sex differences, and the impact of stress.
  • To validate the utility of rank plot analysis for studying GH dynamics in mouse models.

Main Methods:

  • Developed a rank plot analysis method to determine GH concentration from randomly obtained spot samples in large mouse groups.
  • Collected samples rapidly (within seconds) to minimize stress-induced alterations in GH levels.
  • Correlated plasma GH levels with GH receptor/Janus kinase 2/signal transducer and activator of transcription 5 (JAK2/STAT5) pathway activation in the liver.

Main Results:

  • The nadir-to-peak distribution of plasma GH concentration in mice mirrors that of other mammals.
  • Significant nycthemeral (daily) and sex-based differences in GH secretion were observed.
  • Handling stress was identified as a potent immediate downregulator of circulating GH.
  • GH receptor signaling activation correlated with plasma GH levels, but not further increased by peak concentrations.
  • Rank plot analysis applied to GH-deficient brain-specific IGF-1 receptor knockout mice revealed extended trough periods and rare peaks.

Conclusions:

  • Rank plot analysis provides a straightforward and effective method for studying endogenous GH levels and secretion patterns in mice.
  • This method overcomes previous limitations in serial sampling, enabling more robust research into GH physiology and pathophysiology in mice.
  • The findings highlight the importance of rapid sampling and reveal key regulatory aspects of GH secretion, including stress effects and circadian rhythms.