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Updated: Jun 7, 2026
Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
Successful Descemet stripping endothelial keratoplasty in congenital hereditary endothelial dystrophy
Vikas Mittal1, Ruchi Mittal, Virender S Sangwan
1Cornea and Anterior Segment Services, Sanjivni Eye Care, Model Town, Ambala City, Haryana, India. vikas_mittal@hotmail.com
Purpose:
To report successful Descemet stripping endothelial keratoplasty (DSEK) in a patient with congenital hereditary endothelial dystrophy (CHED).
Methods:
A 19-year-old boy presented with complaints of decreased vision, hazy corneas, and nystagmus since birth. Six months prior, the right eye had undergone penetrating keratoplasty. In the left eye, he was planned for DSEK.
Results:
Successful Descemet membrane (DM) scoring and stripping was achieved with a reverse Sinskey hook after trypan blue staining. Manually dissected posterior stromal donor tissue was transplanted using Busin glide. Corneal edema resolved completely with a final best-corrected visual acuity of 20/100 at 6 months. Serial anterior segment optical coherence tomography scans showed maximum decrease in stromal thickness in the first month. Despite resolution of corneal edema, mild stromal haze persisted, which decreased with time. Histopathology of removed DM showed thickened DM and absence of endothelial cells which was suggestive of CHED.
Conclusions:
To the best of our knowledge, this is the first reported case of successful DSEK in a patient with CHED.
