Related Experiment Video
Updated: Jun 7, 2026
Rodent Model of Intestinal Ischemia-Reperfusion Injury via Occlusion of the Superior Mesenteric Artery
Published on: October 20, 2023
Ketamine reduces intestinal injury and inflammatory cell infiltration after ischemia/reperfusion in rats
Francisco Javier Guzmán-De La Garza1, Carlos Rodrigo Cámara-Lemarroy, Raquel Guadalupe Ballesteros-Elizondo
1Departamento de Fisiología, Universidad Autónoma de Nuevo León, Facultad de Medicina, Av. Francisco I Madero y Dr. Eduardo Aguirre Pequeño S/N, Col. Mitras Centro, Monterrey, Nuevo León, 64460, Mexico.
Purpose:
Intestinal ischemia reperfusion (I/R) induces severe injury and significant mortality. New therapeutic interventions are needed; ketamine is an anesthetic with anti-inflammatory properties, which has shown protective effects on I/R in various organs. This study investigated effects of ketamine on intestinal I/R injury.
Methods:
Male Wistar rats underwent either sham surgery or 30 min of intestinal ischemia followed by 60 min reperfusion. Ketamine pretreatment was administered by intraperitoneal injections at doses of 100, 50, 12.5, or 6.25 mg/kg. The intestinal morphology, mucosal damage, leukocyte infiltration, serum P-selectin, serum intracellular adhesion molecule-1 (ICAM-1), serum antithrombin-III (ATIII), and myenteric ganglion cell structure were evaluated.
Results:
Intestinal I/R led to severe mucosal damage, leukocyte (especially neutrophil) infiltration, P-selectin and ICAM-1 elevations, ATIII depletion, and myenteric ganglion cell morphological alterations. The ketamine dose dependently diminished these alterations (except for ICAM-1 serum levels), reaching statistical significance at 100, 50, and 12.5 mg/kg.
Conclusions:
Ketamine protects the intestine against I/R injury. Ketamine anesthesia has been recommended for clinical situations of sepsis and hemodynamic instability, both frequent during intestinal I/R. The clinical application of ketamine in situations of intestinal I/R warrants consideration.
