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[Determination of aurantio-obtusin and chrysophanol in cassiae semen by HPLC]
Xiaoping Huang1, Guoyue Zhong, Xuemei Zhang
1Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
Objective:
To improve the determination method of Cassiae Semen in China Pharmacopeia edition 2005. MMETHOD: Aurantio-obtusin and chrysophanol were separated on an Inertsil ODS-3 column with acetonitrile-0.1% phosphoric acid solutiona as mobile phase in gradient elution.
Result:
The linear ranges of aurantio-obtusin and chrysophanol were 2.3-230, 2.87-286 mg x L(-1), respectively. The average recover were 100.3% (RSD 3.1%), and 99.5% (RSD 2.5%) respectively.
Conclusion:
This method is specific, accurate and reproducible, and can be used for the assay of Cassiae Semen in the new edition of Chinese pharmacopoeia.
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