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[Simultaneous determination of multicomponent in Xuefuzhuyu granule with different UV-wavelength by HPLC]
Zhenghui Liu1, Tingxiang Ye, Linlin Zhao
1Tianjin High-Standard Quality Testing Lab., Tianjin 300081, China. liuzhenghui_2008@163.com
Objective:
To establish the method for the quality control of Xuefuzhuyu granule.
Method:
The content of Semen Armeniacae Amarum PE, Hydroxysafflor yellow A, paeoniflorin and ferulic acid in Xuefuzhuyu granule were determined by HPLC under multi-wavelength of 210, 403, 230, 316 nm.
Result:
The calibration curve of Semen Armeniacae Amarum PE. hydroxysafflor yellow A, paeoniflorin and ferulic acid were linear within 0.1154-0.9232 (r = 0.9998), 0.0219-0.1754 (r = 0.9994), 0.3952-3.1616 (r = 0.9995), and 0.0426-0.3408 microg (r = 0.9998), respectively. The average recoveries were 101.78% (RSD was 1.7%), 99.60% (RSD was 2.9%), 98.90% (RSD was 2.0%), and 100.31% (RSD was 1.8%), respectively.
Conclusion:
The method of quantification is accurate, rapid and reliable for the quality control of Xuefuzhuyu granule.
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