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Quantitative detection of glyphosate by simultaneous analysis of UV spectroscopy and fluorescence using DNA-labeled

Hee Uk Lee1, Hyun Yong Shin, Jin Young Lee

  • 1Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701, Korea.

Journal of Agricultural and Food Chemistry
|November 5, 2010
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...